Evaluation of the discriminatory power of variable number of tandem repeat (VNTR) typing of Mycobacterium bovis isolates from southern Africa Hlokwe,
نویسندگان
چکیده
The usefulness of variable number tandem repeat (VNTR) typing based on limited numbers of loci has previously proven inferior compared to IS6110-RFLP typing when applied to the study of the molecular epidemiology of bovine tuberculosis in both livestock and wildlife in southern Africa. In this study, the discriminatory power of 29 published VNTR loci in the characterization of 131 Mycobacterium bovis strains isolated predominantly from wildlife and a smaller number from livestock in southern Africa was assessed. Allelic diversities calculated when loci were evaluated on a selected panel of 23 M. bovis isolates with identified varying degrees of genetic relatedness from different geographic origins as well as M. bovis BCG ranged from 0.00-0.63. Of the 29 loci tested, 13 were polymorphic (Qub-11a,
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